Fig 6. The promoter for ICP6 drives protein expression during latency.

Groups of ROSA26 mice were infected with HSV-1 pICP6_eGC by tattoo on the flank and were culled at 5, 10, 20 and 40, or 10, 20, and 100 days p.i. for determination of the number of β-gal⁺ cells per DRG. (A) Representative photomicrographs of DRG at spinal levels T11, T10, and T9 of a single mouse for each day p.i. taken at 40× magnification (scale bar = 300 μm, as indicated on top left image). (B) The total number of β-gal⁺ cells per mouse and (C) the spread of virus as indicated by the number of DRG containing β-gal⁺ cells was determined. The data were pooled from six independent experiments, with each point representing a single mouse and the bar representing the mean cell count (n = 11–24 per day p.i.). Differences between groups were assessed using Kruskal Wallis test with Dunn’s posttest for pairwise comparison (**p < 0.01, ***p < 0.001; red denotes differences of particular note).