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. 2016 Feb 24;115(6):2852–2866. doi: 10.1152/jn.01056.2015

Fig. 1.

Fig. 1.

A transgenic GCaMP6s reporter mouse. A: tetO construct used to generate transgenic mouse line. Ptight, Ptight Tet-responsive promoter; SV40, Simian virus 40. B: isolated brain from a GCaMP6s mouse crossed to a CAMK2 driver, viewed under a fluorescence dissecting scope, demonstrating strong expression restricted to cortex. Scale bar, 5 mm. C: low-magnification coronal view, showing broad expression throughout cortex. Scale bar, 2 mm. D: high-magnification coronal view, showing expression throughout the layers of cortex. Scale bar, 500 μm. E: confocal section showing that the majority of neurons expresses GCaMP6s, with negligible filling of cell nuclei. Blue, 4′,6-diamidino-2-phenylindole (DAPI) counterstain. Scale bar, 50 μm.