Fig. 2.

Widefield imaging of neural activity. A: diagram of imaging and head-fixation setup. B: widefield fluorescence image of GCaMP6s fluorescence in a cranial window. Inset shows positioning of cranial window. C: fluorescence trace from a single, 26-μm pixel after blue − green subtraction, showing large step increases in fluorescence locked to timing of a 5-s on, 5-s off noise stimulus. D: cycle-averaged traces from the data in C, before (green) and after (black) temporal deconvolution, showing agreement with multiunit electrophysiological recording (blue). Stimulus duration shown in gray. sp/sec, spikes per second.