Fig. 8.

Two-photon recording of passive visual responses in V1. A: baseline fluorescence image of a typical field of view in upper layer 2/3 of V1. B: pixel-wise map of orientation selectivity as measured with drifting gratings of 2 spatial frequencies and 8 orientations, showing a large number of responsive cells, with nearly all showing orientation selectivity. Color wheel shows preferred orientation (hue) and selectivity (saturation). Scale bars, 50 μm. C: histogram of visually evoked dF/F for all cells recorded (n = 479 cells in 3 mice). D: histogram of orientation selectivity for all visually responsive cells (n = 353 cells in 3 mice). Error bars represent bootstrapped confidence intervals.