Fig. 9.

Targeted 2-photon recording in area 39 during spontaneous locomotion. A: overlaid widefield mapping of response to visual white-noise stimulus. Scale bar, 1 mm. B: widefield mapping of activity during spontaneous locomotion, superimposed on an image of the vasculature, used to identify region for 2-photon recording. Scale bar, 1 mm. C: pixel-wise correlation map between fluorescence traces and locomotion, showing neurons positively correlated with locomotion (red) and negatively correlated (blue). Scale bar, 250 μm. D and E: example fluorescence and locomotion traces from 2 neurons in C, showing activation correlated with locomotion (D) and activation correlated with stationary periods (E). F: histogram of correlation between fluorescence and locomotion for individual cells, with green representing cells that are significantly positively correlated and red representing cells that are significantly negatively correlated (n = 284 cells in 3 animals).