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. Author manuscript; available in PMC: 2016 Jun 27.
Published in final edited form as: Neurotoxicology. 2013 May 30;38:1–8. doi: 10.1016/j.neuro.2013.05.015

Table 2.

Effects of L-methionine (L-Met) on total mercury concentration in C6 cells treated with MeHg, EtHg and their cysteine-complexes (MeHg-Cys and EtHg-Cys).

Mercurial L-Met Total mercury concentration (ng/μL)
MeHg MeHg-Cys EtHg EtHg-Cys
+ 7.28 (0.274)* 5.12 (1.101)* 5.35 (0.274)* 3.72 (0.357)*
+ + 7.87 (1.101)* 2.08 (0.606)*,# 5.96 (0.523)* 2.71 (0.165)*,#
0.0098 (0.0027)
+ 0.0118 (0.0035)

Rat C6 glioma cells were pre-treated with 7 mM L-Met for 15 min before treatment with MeHg, EtHg, MeHg-Cys or EtHg-Cys (7 μM) in HBSS. After the exposure to mercurials (30 min), cells were washed twice with HBSS and, after 30 min, harvested in 200 μL Milli-Q water for mercury quantification. Data are expressed as ng Hg/μL of sample and represented as mean ± SD (N = 2 independent experiments).

*

Significantly different (p < 0.05) from control cells (no mercurial treatment) by two-way ANOVA followed by the Tukey’s HSD test.

#

Significantly different (p < 0.05) from cells treated only with the respective mercurial (no L-Met treatment) by two-way ANOVA followed by the Tukey’s HSD test.