Table 2.
Mercurial | L-Met | Total mercury concentration (ng/μL)
|
|||
---|---|---|---|---|---|
MeHg | MeHg-Cys | EtHg | EtHg-Cys | ||
+ | − | 7.28 (0.274)* | 5.12 (1.101)* | 5.35 (0.274)* | 3.72 (0.357)* |
+ | + | 7.87 (1.101)* | 2.08 (0.606)*,# | 5.96 (0.523)* | 2.71 (0.165)*,# |
− | − | 0.0098 (0.0027) | |||
− | + | 0.0118 (0.0035) |
Rat C6 glioma cells were pre-treated with 7 mM L-Met for 15 min before treatment with MeHg, EtHg, MeHg-Cys or EtHg-Cys (7 μM) in HBSS. After the exposure to mercurials (30 min), cells were washed twice with HBSS and, after 30 min, harvested in 200 μL Milli-Q water for mercury quantification. Data are expressed as ng Hg/μL of sample and represented as mean ± SD (N = 2 independent experiments).
Significantly different (p < 0.05) from control cells (no mercurial treatment) by two-way ANOVA followed by the Tukey’s HSD test.
Significantly different (p < 0.05) from cells treated only with the respective mercurial (no L-Met treatment) by two-way ANOVA followed by the Tukey’s HSD test.