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. Author manuscript; available in PMC: 2016 Jun 27.
Published in final edited form as: Transl Res. 2015 Sep 18;166(6):554–567. doi: 10.1016/j.trsl.2015.09.004

Figure 2. Adipose-derived mesenchymal stem cells (ASCs) isolated from 4 month and 22 month old male C57BL/6 mice demonstrate adipogenic and osteogenic differentiation potential.

Figure 2

The mouse mesenchymal stem cell functional identification kit (R&D Systems, Minneapolis, MN) was used to stimulate differentiation of ASCs into adipocytes (left) and osteoblasts (right), per the manufacturer’s instructions. Briefly, adipocytes were stained using goat anti-mouse FABP4 primary antibody, and osteocytes were stained using goat anti-mouse osteopontin primary antibody. Subsequently, both were exposed to donkey anti-goat IgG affinity purified PAb NorthernLights 557 fluorochrome-labeled secondary antibody (R&D Systems). Immunocytochemical staining of differentiated cells are presented with DAPI (blue) and NorthernLights (red) fluorescence imaging at magnification 40x.