Skip to main content
. 2016 Jun 27;10(6):e0004806. doi: 10.1371/journal.pntd.0004806

Fig 3. mRNA levels of Th1 and Th2 cytokines secreted by stimulated PBMCs.

Fig 3

Total RNAs from PBMCs stimulated by mixtures of ESP and HBV positive serum, HBV positive serum only, or ESPs only were extracted for reverse transcription using cytokine gene-specific primers for Th1 cytokine (A) including IL-2 and IFN-γ and Th2 cytokines (B) including IL-4, IL-6 and IL-10 and human β-actin. The relative expression of each cytokine was detected by quantitative real-time RT-PCR and normalized relative to β-actin expression. Medium only served as a control. Data are shown as the mean ± SEM (*p <0.05, ** p <0.01, *** p <0.001).