. 2016 Jun 27;11(6):e0158401. doi: 10.1371/journal.pone.0158401

Table 1. Primers for RT-PCR.

Gene (Accession No.)	PCR	Primer Sequences	Annealing temperature (°C)	Cycle numbers (cycles)	Product size (bp)
Pap (NM_001134901)	1^st	F: `5’-TCTGAGGATCGGTTGCTATACCTG-3’`	46	40	697
	1^st	R: `5’-GCACTTGGTGGTTGCTTGTG-3’`	46	40	697
	2^nd	F: `5’-GACTGTCCTCGCTTTCAAGAACTC-3’`	46	40	642
	2^nd	R: `5’-CATACACTCTGTGGCCCAGT-3’`	46	40	642
Nt5e (NM_021576)	1^st	F: `5’-CACACCAACACCTTTCTCTACACAG -3’`	50	40	606
	1^st	R: `5’-CTGGCCATATCGATGCACAC-3’`	50	40	606
	2^nd	F: `5’-ACCCATTCATAGTCACCTCTGACG-3’`	50	40	511
	2^nd	R: `5’-TTCTTCAGGGTGGACCCTTTC-3’`	50	40	511
Ntpdase2 (NM_172030)	1^st	F:`5’-AGAGACAGACATGCTAGCACACCAC-3’`	55	15	634
	1^st	R: `5’-TGTTGAAGAGCCGAGAGACGAG-3’`	55	15	634
	2^nd	F: `5’-TTGAGGCAGTGACACAGACG-3’`	55	38	506
	2^nd	R: `5’-GGTAGCATTGCTGGTTCCTGAC-3’`	55	38	506
Plcb2 (NM_053478)	1^st	F: `5’-AAGGCATATCTGAGCCAAGG-3’`	52	25	535
	1^st	R: `5’-TTGCAAGGTGACAGGCACTG-3’`	52	25	535
	2^nd	F: `5’-GGACGATGAAACCTCAATAGCC-3’`	52	40	475
	2^nd	R: `5’-GGCAGCTTCTACACGTTTGC-3’`	52	40	475
Gust (NM_173139)	1^st	F: `5’-TAGTTCAGAGAGCAAGGAGTCAGC-3’`	55	15	520
	1^st	R: `5’-CCGGGAATGTAGAACGTCTTG-3’`	55	15	520
	2^nd	F: `5’-AAGAACTGGAGAAGAAGCTTCAGG-3’`	55	38	366
	2^nd	R: `5’-AGGCTTGAATTCCTGGATCG-3’`	55	38	366
Aadc (NM_012545)	1^st	F: `5’-CACATCTGATCAGGCACATTCCTC-3’`	52	40	683
	1^st	R: `5’-TTAGCCGGAAGCAGACCAAC-3’`	52	40	683
	2^nd	F: `5’-AGCAATTCCTTCAGATGGCAAC-3’`	52	40	537
	2^nd	R: `5’-TGAGACAGCTTCACGTGCTTTC-3’`	52	40	537
Kcnq1 (NM_032073)	1^st	F: `5’-CAGCCTCACTCATCCAGACTGC-3’`	52	25	511
	1^st	R: `5’-ACATCTCGCACATCGTAGGG-3’`	52	25	511
	2^nd	F: `5’-CTATGCTGCGGAGAATCCTGAC-3’`	52	40	456
	2^nd	R: `5’-CGTGCTTGCTGGAATTTCTTC-3’`	52	40	456
Gapdh (NM_017008.4)	1^st	F: `5’-TCATTGACCTGAACTACATGGTC-3’`	55	40	567
Gapdh (NM_017008.4)	1^st	R: `5’-CGTTCAGCTCTGGGATGAC-3’`	55	40	567