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. 2016 Jun 20;126(7):2757–2772. doi: 10.1172/JCI86114

Figure 2. RBPJ is highly expressed in BTICs.

Figure 2

(A) Cleaved NOTCH1 levels were assayed by immunoblot in matched BTICs and non-BTICs isolated from patient-derived xenografts (387, 3691, and 4121). (B) RBPJ mRNA levels in BTICs and non-BTICs were detected by qPCR. Data are displayed as mean ± SEM (t test, **P < 0.01, n = 3). (C) RBPJ protein levels were assayed in matched BTICs and non-BTICs isolated from patient-derived xenografts (387, 3691, and 4121). (D) RBPJ protein levels were assayed by immunoblotting during a time course of BTIC differentiation induced by 10% serum. (E) Coexpression of RBPJ and BTIC markers, SOX2 and OLIG2, was assayed by immunofluorescence in tumorspheres isolated from patient-derived xenografts (3691 and 4121). Nuclei were visualized by DAPI staining. Scale bars: 50 μm. (F) Coexpression of RBPJ and BTIC markers, SOX2 and OLIG2, was assayed by immunofluorescence in two primary human glioblastoma (GBM) specimens (CCF1167 and CCF1265). Nuclei were visualized by DAPI staining. Scale bars: 10 μm.