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. 2016 Jun 20;126(7):2757–2772. doi: 10.1172/JCI86114

Figure 3. Targeting RBPJ decreases BTIC growth and self-renewal.

Figure 3

(A and B) Effects of shRNA against RBPJ were tested in two BTIC models (3691 and 4121). Top: BTICs were transduced with a control, nontargeting shRNA sequence (shCONT) or one of two nonoverlapping shRBPJ sequences. Proliferation was measured by CellTiter-Glo (2-way ANOVA: **P < 0.01, ***P < 0.001, n = 4). Bottom: RBPJ protein levels were assayed by immunoblot following transduction with shCONT or shRBPJ. (C and D) Tumorsphere formation efficiency was measured by extreme in vitro limiting dilution assays in two BTIC models (3691 and 4121) after transduction with shCONT or shRBPJ. (E) Left: Representative images of tumorspheres derived from two BTIC lines transduced with shCONT or shRBPJ are shown. Scale bars: 100 μm. Right: Quantification of tumorsphere size is displayed as mean ± SEM (2-way ANOVA, *P < 0.05, n = 3).