Figure 9. Infusion of AAV2^M1shRNA in mPFC of Sst-Cre mice blocked the antidepressant-like effects of scopolamine compared with scrambled control.

Sst-Cre mice received bilateral infusion of AAV2^M1shRNA or AAV2^SCR in the mPFC. After 3 weeks, mice were tested for baseline behavioral changes, then received saline or scopolamine, followed by additional behavioral tests. (A) Schematic showing experimental approach and time line. Following repeated doses of scopolamine, Sst-Cre mice were tested in FST, FUST, and NSFT. Time spent immobile in the FST (B) and interaction time in FUST (C) are shown. Bars represent the mean ± SEM, n = 5–6/group. *P < 0.05, means significantly different from respective saline group. Numbers in the bars represent the total sample size for each group.