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. 2016 Jun 20;126(7):2689–2705. doi: 10.1172/JCI84658

Figure 4. CKAP4 forms a complex with PI3K.

Figure 4

(A) Lysates (Input) of control MDCK, MDCK/DKK1-FLAG, control X293T, or X293T/DKK1-FLAG cells were immunoprecipitated with anti-CKAP4 antibody or nonimmune IgG and probed with the indicated antibodies. (B) Lysates (Input) of control MDCK, MDCK/DKK1-FLAG, control X293T, or X293T/DKK1-FLAG cells were immunoprecipitated with anti-CKAP4 antibody or nonimmune IgG and probed with the indicated antibodies. (C) P85α was expressed with CKAP4-HA or CKAP4-ECD-HA in X293T/DKK1-FLAG cells, and cell lysates were immunoprecipitated with anti-HA antibody or nonimmune IgG and probed with anti-p85α and anti-HA antibodies. (D) Amino acid sequence of CKAP4 cytoplasmic region. (E) Lysates of X293T/DKK1-FLAG cells transiently transfected with CKAP4-HA or PA mutant–CKAP4-HA were immunoprecipitated with anti-HA antibody or nonimmune IgG and probed with anti-p85α and anti-HA antibodies. (F) Lysates of X293T/DKK1-FLAG cells transiently transfected with WT or deletion mutant GFP-p85α were immunoprecipitated with anti-CKAP4 antibody or nonimmune IgG and probed with anti-GFP and anti-CKAP4 antibodies. (G) Schematic illustration of human p85α deletion mutants used in this study.