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. 2016 Jun 20;126(7):2689–2705. doi: 10.1172/JCI84658

Figure 7. DKK1-CKAP4 signaling is required for cancer cell proliferation in vitro.

Figure 7

(A and B) S2-CP8 (A) and A549 (B) cells stably expressing control shRNA; DKK1 shRNA; DKK1 shRNA and DKK1-FLAG; CKAP4 shRNA; or CKAP4 shRNA and CKAP4-HA were subjected to the 2D cell proliferation assay. Results are shown as the mean ± SD of 3 independent experiments. (C and D) Left panels: The same cell lines used in A and B were cultured for 5 days in 3D Matrigel. Right panel: The number of spheres per field (n = 5) was counted, and results are expressed as the ratio compared with that of control spheres. **P < 0.01; NS, not significant (compared with the shControl group, 2-tailed Student’s t test). Scale bars: 200 μm.