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. 2016 May 23;126(7):2452–2464. doi: 10.1172/JCI82956

Figure 8. SF-1 controls Tlx1 and RA metabolism.

Figure 8

(A) Expression of Tlx1, Cyp26b1, and Vegf-a in E14.5 Sf-1+/+ and Sf-1–/– embryonic spleens. Data are representative of 1 of 2 independent experiments with 4 pooled spleens for each genotype. (B) Luciferase activity on a Cyp26b1 promoter reporter system was assessed at 48 hours in HEK293 cells transiently transfected with increasing concentrations of Sf-1 expression vector. Data are representative of 1 of 3 different experiments. (C) RARE-F9 cells transiently expressing Sf-1 or control vector were treated with RA and expression of indicated genes analyzed by qPCR 8 hours later. Data are representative of 1 of 3 independent experiments. (A–C) The means of triplicates ± SD are shown, *P < 0.05 (A and C), **P < 0.01 (A–C), ***P < 0.001 (B and C) (2-tailed Student’s t test).