Figure 2. Assessment of the renin-angiotensin system in mice overexpressing sFlt1.

(A and B) ELISA of plasma renin (A) and Ang II (B) from gd17 CMV-null (n = 14) and sFlt1 (n = 9) pregnant mice. Unpaired 2-tailed t test; *P < 0.05. (C and D) Gene expression analysis of renal tissue (C) and mesenteric vessels (D) obtained from CMV-null and sFlt1 mice as nonpregnant (NP), gd14, gd17, and postpartum (PP). mRNA analysis (quantitative RT-PCR) was completed for the Ang II receptors (at1ra, at1rb, and at2r). All data were normalized to β-actin (ΔCt). Comparisons between groups of NP, gd14, gd17, and postpartum (PP) mice revealed no differences. Nonpregnant mice were injected with adenovirus at least 7 days before collection. n = 3–8 mice per time point were assayed per tissue. All data represent the mean ± SEM.