Figure 7. Phospho-eNOS and sFLT1 expression in human placentas from normotensive and preeclamptic women.
(A and B) IHC of placentas obtained from preeclamptic women show decreased phospho-eNOS expression (A) and increased sFLT1 expression (B) in the syncytiotrophoblast layer compared with placentas from normotensive pregnancies. Bright-field microscopy images, representative of n = 6 per group. Scale bar: 50 μm. (C) Quantitation of phospho-eNOS immunoreactivity in placentas from uncomplicated and preeclamptic placentas. OD per area (pixels2) of tissue was calculated in 4 high-power fields per sample (n = 6 per group). *P < 0.001, presented as the mean ± SEM. Analyzed by 2-way ANOVA. (D) Phospho-eNOS immunoreactivity correlates negatively with sFLT1 immunoreactivity in the placenta. Analyzed by Pearson’s correlation, P < 0.001.