Skip to main content
. 2016 Jun 13;126(7):2626–2641. doi: 10.1172/JCI84637

Figure 5. Notch activation promotes both proliferation and apoptosis of Pten-null prostate cancer cells.

Figure 5

(A and B) Coimmunostaining of Ki-67 and K8 (A) and cleaved caspase 3 (CC3) and K8 (B) in 16-week-old PB-Pten and PB-Pten-NICD mouse prostates. Bar graphs show means ± SD of Ki-67+ and CC3+ cells in 3 mice per group. ***P < 0.001 by Student’s t test. (C) Coimmunostaining of pH2AX and K8 in 16-week-old PB-Pten and PB-Pten-NICD mouse prostates. (D) Western blot analysis of pSer15 p53 and total p53 in prostate tissue lysates from 16-week-old PB-Pten and PB-Pten-NICD mice. (E) qRT-PCR analysis of 3 p53 target genes in prostate tissues of 16-week-old PB-Pten and PB-Pten-NICD mice. n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test. Scale bars: 50 μm.