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. 2016 Jun 13;126(7):2626–2641. doi: 10.1172/JCI84637

Figure 8. Notch promotes epithelial-mesenchymal transition.

Figure 8

(A) Heat map of expression of EMT-related genes in prostate tissue of PB-Pten mice (PB-Pten) and PB-Pten-NICD mice (PB-Pten-NICD), 2 prostate-derived lung metastases (lung metastatic) from PB-Pten-NICD mice (nos. 4 and 5 in Figure 7C), and 2 cell lines (PtNI) established from prostate primary and metastatic prostate tumors of PB-Pten-NICD mice. (B) qRT-PCR analysis confirms differential expression of 8 genes. Bar graphs show means ± SD of gene expression in prostate tissues of PB-Pten and PB-Pten-NICD mice (n = 12 each), and the PtNI-Met cell line established from metastatic prostate tumors in PB-Pten-NICD mice (9 independent assays on the same cell line). *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA with Dunnett’s correction for multiple comparisons. (C) Western blot analysis of fibronectin (FN1), FOXC2, vimentin (Vim), Snail, Slug, AKT, and MAPK in prostate lysates of 16-week-old PB-Pten and PB-Pten-NICD mice. Each lane represents an independent specimen. NRAEV, normalized relative arbitrary expression value by β-actin. (D) Coimmunostaining of vimentin and α-smooth muscle actin (SMa) in prostate tissues of 16-week-old PB-Pten and PB-Pten-NICD mice. Scale bars: 50 µm.