Figure 1.

Structure and amyloidogenicity of mβ₂m. (A) The NMR structure of monomeric hβ₂m (gray-2XKS²⁸) overlaid with the crystal structure of mβ₂m bound to the MHC-I complex (green-1LK2³⁸) or with the solution structure of ΔN6 (red-2XKU²⁸). (B) Aggregation assay of 80 μM hβ₂m (gray), mβ₂m (green), or ΔN6 (red) in 10 mM sodium phosphate buffer, pH 6.2 (three replicates for each protein). Negative stain electron micrographs, color-coded with the same scheme, are shown on the right (scale bar = 500 nm). (C) The ¹H–¹⁵N HSQC spectrum of mβ₂m in the same buffer as (B). (D) Correlation between experimental RDCs measured for mβ₂m in 10 mg/mL phage PF1 and those back-calculated from the crystal structure 1LK2³⁸ (R² = 0.85).