Figure 2.

Ex vivo immunohistology of human skin explants assessing the potential of GGO for restoring the number of fibrillin-1 immunoreactive fibers.

Notes: Human skin explants were cultured at 37°C and at a CO₂ pressure of 5% in a standard cell culture medium and incubated or not with methylglyoxal at 500 μM for 8 days to induce glycation. Certain samples were concomitantly treated with GGO at 10 μM. Dashed arrows show fibroblasts, solid arrows show fibrillin-1 fibers. Fibrillin-1 was detected by incubating tissue sections with monoclonal antibodies. Immunostaining on a Masson trichrome counterstained tissue and visualization of fibrillin-1 were performed using the streptavidine/peroxidase method (A, C, and E). Nuclei were stained using propidium iodide. Tissue slides of 5 μm were stained with Bouin (B, D, and F).

Abbreviation: GGO, glycylglycine oleamide.