Figure 2.2.

Example of lipid titration measurement using FCS performed outside of the “infinite dilution regime.” Correlation curves (symbols) were measured for samples containing 1 nM ANX and no LUV or LUV with 1, 2, and 4 μM of total lipid LUV (bottom-to-top). Progressive vesicle binding of fluorescently labeled ANX results in a reduction of the number of fluorescent particles in the focal volume and a corresponding increase in total autocorrelation amplitude. Solid curves correspond to fit with Eq. (2.3) (or Eq. (2.1), for sample containing no LUV). The fractions of bound ANX f_b are calculated by comparing absolute amplitudes in the presence and absence of LUV (Eq. (2.6)). Reproduced from Posokhov et al. (2008a) with permission.