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. 2016 May 31;49(2):499–508. doi: 10.3892/ijo.2016.3548

Figure 4.

Figure 4

Effects of TGF-β receptor inhibitor and IL-1 receptor antagonist on KCOT fluid-induced RANKL expression. The TGF-β receptor inhibitor (1 μM) and IL-1 receptor antagonist (50 ng/ml) were used to pretreat KCOT stromal fibroblasts for 1 h. These KCOT stromal fibroblasts were then cultured for 12 h in the combined presence of IL-1α (1 ng/ml) and TGF-β1 (1 ng/ml) or 1% KCOT fluid (with or without acid treatment as indicated by KCOT acid fluid), and RT-PCR was used to examine RANKL expression. The increase in RANKL expression induced by the combination of TGF-β1 and IL-1α was approximately equal to the increase induced by intracystic fluid. Intracystic fluid-induced RANKL expression was partially inhibited by the IL-1 receptor antagonist, strongly inhibited by TGF-β receptor inhibitor, and completely inhibited by a combination of the two.