Figure 3.

Paracrine effects of lineage-specific SMCs on HUVEC networks. HUVECs were exposed to supernatant conditioned by lineage-specific SMCs (supplemental online Fig. 4B). (A): Confocal images of paracrine cocultures taken on days 4 and 8 of the protocol. (B): Quantification of total network area. (C): Quantification of total cord length. (D): Quantification of number of branch points. Quantitative data are shown for day 4 of the coculture (∗, p < .05; ∗∗, p < .01; n = 3 independent biological replicates; scale bars = 100 μm). Abbreviations: d, day; HUVEC, human umbilical vein endothelial cell; LM, lateral mesoderm; NE, neuroectoderm; PM, paraxial mesoderm; SMC, smooth muscle cell.