Figure 3.

Quantitative reverse-transcription polymerase chain reaction analysis of gene expression for forebrain, hindbrain, and corticogenesis markers in undifferentiated human induced pluripotent stem cells, day 14 cerebral organoids (cOrgs), and day 28 cOrgs. Values indicated are mean ± SD. Data are given for gene expression for each individual cell line at D0 (undifferentiated induced pluripotent stem cell), D14, and D28, and the “overall” analysis included pooled data from all four cell lines (WT1, WT3, ALD1, and ALD3) at each time point. Abbreviations: DCX, doublecortin; EGR2, early growth response 2; FOXG1, Forkhead box G1; ISL2, islet 2; SIX3, sine oculis homeobox 3.