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. 2016 Jun 28;7:949. doi: 10.3389/fpls.2016.00949

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Copyright © 2016 Bergau, Navarette Santos, Henning, Balcke and Tissier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence activated cell sorting (FACS) histograms of the trichome fraction harvested in sorbitol-buffer. (A) Preselection of young type VI trichomes in region P1 by the height of the forward scatter (FSC-H) and the area of the forward scatter (FSC-A). This allows the discrimination of single trichome heads and aggregates. The P1 region was selected for further separation based on differential fluorescence as shown in (B). (B) Separation of young type VI trichomes (region P2) and mature type VI trichomes (region P3) based on different ratios between red (PerCP channel) and green (AlexaFluor 488 channel) fluorescences. (A,B) The units are arbitrary units provided by the instrument.