Figure 2.

Exemplar gross, histological, and radiological examinations demonstrate precise and accurate gray and white matter transections and no recording-site tissue abnormalities. (A) Magnified gross specimen of recording site shows gray (g) and white (w) matter separated by dotted-line. Illustration (bottom-left) shows orientation and white bar represents 1 mm. Microscopy of functionally-disconnected cortex stained with hematoxylin and eosin stain confirms white (B) and gray (C) matter disruptions. Asterisk indicates edge of white matter transection; Double asterisk indicates edge of gray matter disruption. (D) T1- and (E) T2-weighted magnetic resonance imaging radiology of recorded neocortex demonstrates no abnormalities. Inset demonstrates magnified recording area and triple-asterisk indicates left side. (F) Low magnification microscopy of middle temporal gyrus recording location stained with hematoxylin and eosin fails to demonstrate any causes or sequela of epilepsy or other pathology. Higher magnification microscopy of gray (G,H) and white (I,J) matter confirm no abnormalities in microscopic laminar architecture and cellular morphology.