FIG. 3.

Effects of glycinecin A and nigericin on the ΔpH of X. campestris pv. vesicatoria (A) and E. coli (B) cells, as indicated by the efflux of the fluorescent dye BCECF. BCECF-loaded and glucose-energized cells were diluted in 50 mM potassium phosphate buffer. Valinomycin was added to a concentration of 5 μM at the time indicated by arrow 1 to dissipate ΔΨ, and glycinecin A (5,120 AU) and nigericin (5 μM) were added at the times indicated by arrows 2 and 3, respectively. Data represent results of three independent experiments.