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. 2016 Jun 28;4:29. doi: 10.3389/fchem.2016.00029

Figure 2.

Figure 2

Spinach and HBI and their optimized derivatives. (A) Secondary structure of the RNA aptamer Spinach determined from its crystal structure. The fluorogen (green) is stabilized on top of the G-quadruplex structure. (B) Secondary structure temperature-optimized Spinach2. (C) Deduced secondary structure of Baby Spinach engineered from the crystal structure essential features. (D) Structure of HBI stabilized within GFP, where the gray regions represent the amino acid segments not involved in the fluorescence. (E) Structure of DFHBI, the fluorogens used for most studies in this review. (F) Structure of DFHBI-1T, the fluorogens with spectral properties more adapted to optics used with GFP fluorescence.