Figure 4.

Other efficient light-up aptamers. (A) RNA Mango aptamer predicted secondary structure. (B) Acetylated thiazole orange (TO1), the fluorogen moiety of the Mango ligand. (C) PEGylated biotin, one of the many derivatives essayed, resulting to TO1-biotin, the ligand for RNA Mango. (D) Predicted secondary structure of Broccoli (in green). Effective fluorogens for Broccoli can be DFHBI or DFHBI-1T. The stem-loop in blue is the insertion point to transform this light-up aptamer as a metabolite sensor.