Table 1.

Photophysical and binding properties of fluorophore-aptamer complexes.

Fluorogen	Aptamer	λex (nm)	λem (nm)	εa (M−1cm−1)	φb	KcD (nM)	Brightnessd	References
Aeguorea GFP	–	395	508	27600	0.79	–	21800	Paige et al., 2011
EGFP	–	489	508	55000	0.60	–	33000	Paige et al., 2011
Malachite green	–	618			7.9 × 10−5	–		Babendure et al., 2003
	MGA	630	650	150000	0.19	117	28500	Babendure et al., 2003
Hoechst 1C	–	345	470	N.D.	0.017	–		Sando et al., 2008
	BFR	345	470	N.D.	0.26	35	N.D.	Sando et al., 2008
DIR	–			134000		–		Constantin et al., 2008
	DIT-Apt1	600	~650	N.D.		86	N.D.	Constantin et al., 2008
DMHBI	–	394	487	23336	0.0005	–	12	Paige et al., 2011
	2–4	397	464	21536	0.10	N.D.	21500	Paige et al., 2011
	13–2	398	529	23391	0.06	464	1400	Paige et al., 2011
	13–2-min	398	529	23391	0.11	N.D.	2600	Paige et al., 2011
	3–6	398	537	25300	0.05	406	1300	Paige et al., 2011
	17–3	405	547	18127	0.09	N.D.	1600	Paige et al., 2011
DMABI	–	447	519	14329	0.003	–	40	Paige et al., 2011
	11-3	489	512	18743	0.05	N.D.	900	Paige et al., 2011
2-HBI	–	399	461	10943	0.0005	–	5	Paige et al., 2011
	6–8	396	588	10714	0.004	N.D.	43	Paige et al., 2011
DFHBI	–	405	498	11864	0.0007	–	8	Paige et al., 2011
	Spinach (24–2)	452	496	24271	0.72	537	17000	Paige et al., 2011
	Spinach2	454	498	26100	0.70	430	18000	Strack et al., 2013
TO1-Biotin	–	500	525	N.D.	0.0002	–	N.D.	Dolgosheina et al., 2014
	Mango	510	535	77500	0.14	3.2	11000	Dolgosheina et al., 2014
TO3-Biotin	–							Dolgosheina et al., 2014
	Mango	637	658	N.D.	N.D.	6–8		Dolgosheina et al., 2014
DFHBI-1T	–	426	495	35400	0.00098		35	Filonov et al., 2014
	Spinach2	482	505	31000	0.94	560	29000	Song et al., 2014
	Broccoli	472	507	29600	0.94	360	27800	Filonov et al., 2014
DFHBI-2T	–	460	515	34800	0.0012		50	Song et al., 2014
	Spinach2	500	523	29000	0.12	1300	3000	Song et al., 2014
PFP-DFHBI	–							Ilgu et al., 2015
	Spinach2					160		Ilgu et al., 2015

^aMolar extinction coefficient.

^bFluorescence quantum yield.

^cDissociation constant for the fluorophore-aptamer complex.

^dBrightness is the product between the molar extinction coefficient and the fluorescence quantum yield.

N.D., not determined.