TABLE 2.

SCFA detected in fermentation culture fluid via gas chromatography^a

Substrate	Steady state no.	Sample vessel no.	Total SCFA (mM)	Acetic acid (mM)	Propionic acid (mM)	Butyric acid (mM)
Polydextrose†	1	1	100	29	31	32
		2	135	36	37	50
		3	149	46	36	52
	2	1	183	78	51	48
		2	224	89	59	64
		3	245	104	63	57
Lactitol monohydrate†	1	1	76	32	16	26
		2	107	35	20	42
		3	161	50	30	64
	2	1	190	48	49	89
		2	232	57	53	102
		3	240	63	54	101
Lactitol monohydrate:	1	1	133	40	35	47
polydextrose†		2	156	43	41	57
		3	157	42	40	59
	2	1	173	82	30	54
		2	205	82	41	65
		3	214	80	42	72
Oligofructose†	1	1	129	31	38	49
		2	137	34	39	48
		3	142	37	38	49
	2	1	174	50	48	69
		2	226	79	52	83
		3	250	93	56	86
Polydextrose (parallel)‡	1	1	79	29	3	39
		2	145	64	12	46
		3	126	51	7	51
	2	1	184	69	32	54
		2	259	126	40	55
		3	269	131	43	57
FOS (parallel)‡	1	1	108	43	11	38
		2	151	62	14	45
		3	165	68	15	48
	2	1	126	45	9	60
		2	189	77	20	67
		3	196	82	20	66

^aDetermination of SCFA in the culture fluid of each fermentation vessel was determined at both the first steady state (before addition of the test substrate) and at the second steady state (after the test substrate had been added). A dagger (†) refers to fermentations inoculated with one sample from the same healthy human donor. A double dagger (‡) refers to fermentations inoculated with a pooled sample from four healthy human donors and carried out in parallel.