TABLE 2.
Change in motile colony morphology of TM1040 in response to putative attractants when grown in BM motility agar with different carbon sources
Chemical | Change in morphology with carbon sourcea
|
|||
---|---|---|---|---|
Glycerol | Glucose | Succinate | Alanine | |
Acrylate | ++ | + | ++ | + |
Alanine | ++ | − | + | − |
Alpha-ketoglutarate | − | − | + | − |
DMSO | − | − | − | − |
DMSP | ++ | − | + | + |
Galactose | ++ | − | + | − |
Glucose | + | − | + | + |
Glutamic Acid | ++ | − | + | − |
Lactose | − | − | − | − |
Lysine | + | − | − | − |
Maltose | + | − | ++ | ++ |
N-acetylglucosamine | ++ | − | + | + |
Succinate | + | + | − | ++ |
Valine | ++ | − | + | + |
TM1040 was inoculated into the center of a BM motility agar plate containing a single carbon source (glycerol, glucose, succinate or alanine) and allowed to grow and move outwards. Chemicals were then spotted near the periphery of the motile colony, and the change in colony appearance compared to the control (no spotted chemical) was recorded on a plus/minus scale.