TABLE 3.
Reduction in chemotaxis of TM1040 toward untreated and heated P. piscicida homogenates by external addition of known attractants
| Treatmenta | No. of bacteria per capillary (CFU, 104)b with:
|
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|---|---|---|---|---|---|---|---|
| Controld | Attractant added to external bufferc
|
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| DMSP | % Reductione | Methionine | % Reduction | Valine | % Reduction | ||
| None | 7.24 (± 1.39) | 3.33 (± 0.15) | 54.01 | 3.03 (± 0.32) | 58.15 | 1.53 (± 0.15) | 78.87 |
| Heat | 3.51 (± 0.35) | 3.10 (± 0.16) | 11.68 | 1.80 (± 0.11) | 48.72 | 0.91 (± 0.24) | 74.07 |
a
Chemotaxis of TM1040 toward heated (80°C for 15 min) or untreated P. piscicida homogenates was measured using the capillary assay (see Materials and Methods).
b
Standard deviation is given in parentheses.
c
Prior to the assay, DMSP, methionine, or valine was added to external buffer containing motile cells of TM1040 at a final concentration of 200 μM and mixed to homogeneity.
d
The control was buffer only.
e
% Reduction, the percent reduction in CFU per capillary compared to the control.