Figure 5.

Oxygen tension effect on platelet‐activating factor (PAF) receptor protein expression, studied in control conditions and treatments with cAMP/cGMP analogs. Western blotting was performed on the same gel as described in methods. Receptor protein expression was quantified by protein loading, normalized to total actin expression. Data are mean ± SEM, n = 3 different determinations. At baseline, hypoxia and hyperoxia decreased PAFR expression from normoxia control. In normoxia, treatment with cAMP and cGMP analogs both attenuated PAFR expression, while under hypoxic conditions treatment with cAMP and cGMP analogs augmented PAFR expression. In hyperoxic conditions, no difference was observed among treated and untreated cells. Statistics are: *P < 0.05, different from baseline; ^# P < 0.05, different from normoxia; ⁺ P < 0.05, different from hypoxia.