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Purification of peroxidase from A. aegerita TM A1a
| Purification step | Amt of protein (mg) | Total activity (U) | Sp act (U mg−1) | Yield (%) | Purifi- cation (fold) |
|---|---|---|---|---|---|
| Culture liquid | 465 | 1,380 | 3 | 100 | 1 |
| Ultrafiltration (10 kDa) | 123 | 756 | 6 | 55 | 2 |
| Q Sepharose | 8.2 | 531 | 64 | 38 | 22 |
| SP Sepharose (AaP I) | 0.46 | 43 | 92 | 3 | 31 |
| SP Sepharose (AaP II) | 2.73 | 299 | 110 | 22 | 37 |
| Mono S (AaP I) | 0.12 | 20 | 167 | 1 | 56 |
| Mono S (AaP II) | 1.48 | 244 | 165 | 18 | 55 |
Enzyme activities are based on the oxidation of veratryl alcohol at pH 7.
