TABLE 3.

Oxidation of several alcohols and aldehydes by purified AaP^a

Substrate	Amt of substrate converted (μM)	Identified product(s)
Anisyl alcohol	157	Anisaldehyde
Anisaldehyde	44	NDb
Benzyl alcohol	144	Benzaldehyde, benzoic acid
Benzaldehyde	62	Benzoic acid
Veratryl alcohol	92	Veratraldehyde, veratric acid
Veratraldehyde	11	Veratric acid
Ethanol	0

^aEach reaction mixture (1 ml) consisted of sodium phosphate/citrate buffer (pH 7), 200 μM substrate (alcohols), 15 mM glucose, 0.2 U of glucose oxidase, and 1 U of AaP. The reaction time was 20 min with continuous stirring at 25°C. The substrate concentration was determined by high-performance liquid chromatography or gas chromatography-mass spectrometry (ethanol). Oxidation of intermediately formed aldehydes was calculated by the decrease in the course (20 min) of the enzymatic reaction. The data are means for three parallel experiments; the standard deviation was <5% in all cases.

^bND, not determined.