TABLE 5.
Oxidation of different substrates and halogenation of MCD by purified AaP and CPO from C. fumagoa
| Substrate | Sp act of AaP (U mg−1) | Sp act of CPO (U mg−1) |
|---|---|---|
| ABTS | 295.7 | 1.2 |
| DMP | 99.6 | 1.9 |
| Benzyl alcohol | 234.2 | 18.1 |
| Benzaldehyde | 236.7 | 0 |
| MCD (Cl−) | 71.8 | 1,537 |
| MCD (Br−) | 354.3 | 2,859 |
a
Most reactions were carried out in sodium citrate/phosphate buffer (pH 7); the exceptions were chlorination and bromination of MCD and oxidation of ABTS, which were carried out in phosphate buffer at pH 2.75 and 5.0, respectively. The experiments were carried out in triplicate, and the standard deviations were <5%.