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SDS–PAGE of preparations of strain GUP and control strain pili. OmcS was detected in controls boiled in SDS (designated S–B) of both types of pili preparations. Pili preparations that were dissociated by boiling in SDS at pH 1.5 (designated S–B–A) contained OmcS as well as the PilA monomer of the expected molecular weight for G. urannireducens PilA (20 kDa; designated G.u. PilA) in the GUP strain and G. sulfurreducens PilA (6.6 kDa; designated G.s. PilA) in the control strain.
