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Primer pairs and amplicon lengths for use in MPN-PCR
| Target organism(s) | Primer name | Primer sequence (5′-3′) | Amplicon length (bp) | Reference |
|---|---|---|---|---|
| Anaeromyxobacter sp. | 60F | CGA GAA AGC CCG CAA GGG T | 401 | This study |
| 461R | ATT CGT CCC TCG CGA CAG T | |||
| Paenibacillus Brevibacillus spp. | 160F | TGA GTA ACA CGT AGG CAA CCT | 174 | This study |
| 334R | TAA TGC GCC GCA GGC CCA T | |||
| Geobacter sp. | 494F | AGG AAG CAC CGG CTA ACT CC | 331 | 18 |
| 825R | TAC CCG CRA CAC CTA GTC T |
