TABLE 4.
MPN-PCR assay results
| Borehole set | Organism(s) probed | No. of copies of 16S RNA gene/g of sediment (95% confidence limits)a
|
|
|---|---|---|---|
| Before biostimulation | After biostimulation | ||
| A | Geobacter sp. | 5.38 × 103 (4.23 × 103-2.63 × 104) | 2.63 × 105 (2.19 × 105-8.50 × 105) |
| B | Geobacter sp. | 5.38 × 103 (4.23 × 103-2.63 × 104) | 1.50 × 103 (1.06 × 103-7.38 × 103) |
| C | Geobacter sp. | 2.25 × 103 (1.80 × 103-8.85 × 103) | 1.50 × 103 (1.06 × 103-7.38 × 103) |
| D | Geobacter sp. | 6.50 × 102 (4.25 × 102-1.30 × 103) | 2.88 × 104 (2.38 × 104-1.50 × 105) |
| A | Anaeromyxobacter sp. | 2.63 × 104 (2.10 × 104-1.03 × 105) | 1.16 × 103 (9.75 × 102-5.91 × 103) |
| B | Anaeromyxobacter sp. | 2.63 × 104 (2.10 × 104-1.03 × 105) | 1.13 × 102 (1.00 × 102-5.63 × 102) |
| C | Anaeromyxobacter sp. | 3.45 × 103 (2.25 × 103-1.46 × 104) | 5.38 × 103 (4.50 × 103-1.79 × 104) |
| D | Anaeromyxobacter sp. | 4.25 × 104 (3.18 × 104-1.65 × 105) | 1.16 × 103 (9.75 × 102-5.91 × 103) |
| A | Paenibacillus-Brevibacillus spp. | 1.16 × 103 (9.75 × 102-5.91 × 103) | 1.43 × 102 (9.3 × 101-6.07 × 102) |
| B | Paenibacillus-Brevibacillus spp. | 1.16 × 103 (9.75 × 102-5.91 × 103) | 1.00 × 100 |
| C | Paenibacillus-Brevibacillus spp. | 1.00 × 100 | 6.98 × 103 (5.85 × 103-3.55 × 104) |
| D | Paenibacillus-Brevibacillus spp. | 2.35 × 104 (1.65 × 104-7.85 × 104) | 1.88 × 102 (1.5 × 102-7.38 × 102) |
a
Quantitative PCR assay results obtained before and after biostimulation of contaminated sediment with glucose (comparing FB32 to nearby FB45 [borehole set A], FB32 to nearby FB46 [borehole set B], and FB33 to nearby FB47 [borehole set C]) and ethanol (comparing FB34 to nearby FB49 [borehole set D]) are shown.