Figure 1.

Silencing of PKC-α reduces late endosome formation. (a) HT1080 cells were transduced with a nontargeting shRNA control (Cntr shRNA) or a specific shRNA directed to the PKC-α mRNA (PKCα shRNA). Western analysis demonstrates effective and specific silencing of PKC-α protein expression in the derived stable cell line. The mock control indicates the parental un-transduced cells. α-tubulin was the loading control. (b) Silencing of PKC-α protein expression reduces late endosome formation as indicated by the concomitant reduction of Rab7 protein (a marker for late endosomes) in cells expressing the PKC-α shRNA (PKC-α shRNA), but not the nontargeting shRNA control (Cntr shRNA). Two technical replicates are shown. α-tubulin was the loading control.