Figure 4.

Determination of luciferase activity from tissue lysates. Luciferase transgene expression analysis in tissue lysates 14 days after vector administration. Data are shown as bars (mean) with plotted individual data points (n = 3 animals per group). (a) Organ distribution of luciferase transgene expression mediated by the three vectors as detailed in Figure 3a. (b) Direct comparison of transgene expression mediated by vectors carrying wild-type AAV2 capsids (AAV2-WT), CVGSPCG-displaying control capsids or ESGHGYF-displaying lung-targeted capsids in three organs in which transgene expression could be detected by bioluminescence imaging (heart, liver, and lung).