FIG. 4.

Responses of G. arboreum suspension-cultured cells to His-VdNEP. (A) Induced formation of sesquiterpene aldehydes (gossypol equivalents) in cells treated for 36 h. Lanes: 1, His tag protein, 0.1 μg/ml; 2 to 6, His-VdNEP at 0.01, 0.05, 0.1, 0.5 and 1 μg/ml, respectively; 7, crude elicitors of V. dahliae (1 μg protein/ml). DW, dry weight. (B) Relative increase in cell death estimated by monitoring Evans blue retention by determining the absorbance at 600 nm. (C) DNA laddering induced by His-VdNEP. Lanes: 1, PBS buffer; 2, His tag protein at 0.5 μg/ml; 3 to 5, His-VdNEP at 0.05, 0.1 and 0.5 μg/ml, respectively; M, DL-2000 marker (Takara Biotechnology, Dalian, China).