TABLE 2.

Identification of selected proteins that were differentially expressed by S. enterica serovar Enteritidis ATCC 4931 cells following TSP or alkaline pH pretreatment^a

Protein identification (regulation)b	Protein	Function/family	pI (pH)	Molecular mass (kDa)
A (↓)	Translational elongation factor	Promotes GTP-dependent binding of aminoacyl-tRNA to A site of ribosomes during protein biosynthesis	5.3	43.3
1 (↑)	Arginyl-tRNA synthetase	ATP + l-arginine + tRNA (ARG) = AMP + diphosphate + l-arginyl-tRNA(ARG); belongs to class I aminoacyl-tRNA synthetase family	6.3	72.2
3 (↑)	Arginosuccinate lyase	N-(l-Argino)succinate = fumarate + l-arginine; arginine biosynthesis	5.1	50.8
11 (↑)	Trigger factor	Involved in protein export; acts as chaperone by maintaining newly synthesized protein in open conformation	4.5	48.9
I (↑)	Aspartokinase 2	First step in common biosynthetic pathway leading from ASP to diaminopimelate and LYS	4.8	44.3
II (↑)	OM protein C	Forms passive diffusion pores that allow low-molecular-weight hydrophilic materials to across OM	4.6	41.2
III (↑)	OM protein W	Belongs to OM OMPW/ALKL family	5.6	22.9
IV (↑)	Xanthine phosphoribosyltransferase	Nucleotide metabolism; purine metabolism	5.2	17.4

^aThe protein spots were identified by MS analysis. Function and family analyses were based on search results from the National Center for Biotechnology Information database.

^bProtein identifications correlate with spot and band identifications in Fig. 1 and 2. Arrows in parentheses beside protein identifications indicate whether the proteins were up- or down-regulated relative to the control.