Fig. 1. Effects of resveratrol (RSV) on the differentiation of brown adipocytes. (A) Oil-red-O staining of primary cultured brown preadipocytes. RSV treatment was performed with induction medium at doses of 50 and 100 µM and maintained thereafter. Adipogenesis was significantly increased with RSV treatment. (B) Western blotting for mitochondrial activity and estrogen receptor α (ER-α) in brown adipocytes. Brown preadipocytes were cultured and differentiated with or without RSV treatment. The protein lysates were collected after the differentiation of brown adipocytes was confirmed. UCP-1, uncoupling protein 1; p-AMPK, phospho-AMP-activated protein kinase; AMPK, AMP-activated protein kinase.