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Pioglitazone blocked the DES-induced PPAR-γ ubiquitination and degradation in vivo. (a) PPAR-γ DNA binding activity was shown. (b) PPAR-γ protein was tested by Western blot. (c) The mRNA expression of PPAR-γ. (d) The ubiquitination of PPAR-γ was detected by coimmunoprecipitation assay. ∗ P < 0.05, compared with baseline.
