TABLE 1.
Primer | Nucleotide sequencea | Restriction site |
---|---|---|
YqgB1 | 5′-ACCAAGCCCAACCAGAACC-3′ | |
YqgB2 | 5′-CATGCCATGGTTGTCCTCCGCCGTTTGG-3′ | NcoI |
YqgB3 | 5′-CGGTAAAACCATTTTGTATATTA-3′ | |
YqgB4 | 5′-CCATGCCAACACCATCCTGGAT-3′ | |
YqgB5 | 5′-CGCGGATCCCTAGGAATATATGCTTGTCC-3′ | BamHI |
YqgB6 | 5′-CGCGGATCCGGAACGGCAACGCGGACGAC-3′ | BamHI |
YqgB7 | 5′-AACTGCAGATTCCGTAAAAAAAGCTTCCTTC-3′ | PstI |
YqgB8 | 5′-CGCGGATCCATGCCTACACCATCTTGG-3′ | BamHI |
YqgB9 | 5′-AAACTGCAGGTTCTGCTATGAGCGGTC-3′ | PstI |
YqgB10 | 5′-AAACTGCAGAAAACCTTTTGTATATTA-3′ | PstI |
YqgB11 | 5′-GGAATTCCGGTTCTGCTATGAGCGGTC-3′ | EcoRI |
YqgB12 | 5′-TCCCCCGGGTTCTTGGAATAGTAG-3′ | SmaI |
YqfZ1 | 5′-TCTCGTTCAGCAGCCATTTCAGCA-3′ | |
YqfZ2 | 5′-CATGCCATGGCCTATCGGATATGCTCTTTAAG-3′ | NcoI |
YqfZ3 | 5′-CCGGAATTCGGAAAGTCTTATAAATTCCCG-3′ | EcoRI |
YqfZ4 | 5′-CGCGGATCCGTTTGGGCAGCAGTTG-3′ | BamHI |
YqfZ5 | 5′-TCCCCCGGGCCGAGAACAAGCACGAATAG-3′ | SmaI |
YqfZ6 | 5′-CGCGGATCCACGTGCACCGGCAATACC-3′ | BamHI |
Restriction sites are underlined.