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. 2004 Aug;70(8):4784–4791. doi: 10.1128/AEM.70.8.4784-4791.2004

TABLE 1.

Primer sequences used in this study

Primer Nucleotide sequencea Restriction site
YqgB1 5′-ACCAAGCCCAACCAGAACC-3′
YqgB2 5′-CATGCCATGGTTGTCCTCCGCCGTTTGG-3′ NcoI
YqgB3 5′-CGGTAAAACCATTTTGTATATTA-3′
YqgB4 5′-CCATGCCAACACCATCCTGGAT-3′
YqgB5 5′-CGCGGATCCCTAGGAATATATGCTTGTCC-3′ BamHI
YqgB6 5′-CGCGGATCCGGAACGGCAACGCGGACGAC-3′ BamHI
YqgB7 5′-AACTGCAGATTCCGTAAAAAAAGCTTCCTTC-3′ PstI
YqgB8 5′-CGCGGATCCATGCCTACACCATCTTGG-3′ BamHI
YqgB9 5′-AAACTGCAGGTTCTGCTATGAGCGGTC-3′ PstI
YqgB10 5′-AAACTGCAGAAAACCTTTTGTATATTA-3′ PstI
YqgB11 5′-GGAATTCCGGTTCTGCTATGAGCGGTC-3′ EcoRI
YqgB12 5′-TCCCCCGGGTTCTTGGAATAGTAG-3′ SmaI
YqfZ1 5′-TCTCGTTCAGCAGCCATTTCAGCA-3′
YqfZ2 5′-CATGCCATGGCCTATCGGATATGCTCTTTAAG-3′ NcoI
YqfZ3 5′-CCGGAATTCGGAAAGTCTTATAAATTCCCG-3′ EcoRI
YqfZ4 5′-CGCGGATCCGTTTGGGCAGCAGTTG-3′ BamHI
YqfZ5 5′-TCCCCCGGGCCGAGAACAAGCACGAATAG-3′ SmaI
YqfZ6 5′-CGCGGATCCACGTGCACCGGCAATACC-3′ BamHI
a

Restriction sites are underlined.