TABLE 5.
Km values for FAO1, FAO2a, and FAO2a′
| Substrate |
Km (range [μM])a
|
||
|---|---|---|---|
| FAO1 | FAO2a | FAO2a′ | |
| Octanol | 41.0 (35.0-49.3) | 503 (329.3-1069.2) | 654 (323.5-30,607.9) |
| Decanol | 15.1 (11.8-21.3) | 74 (64.3-85.9) | 69.6 (65.1-74.8) |
| Dodecanol | 14.9 (11.9-19.9) | 2.5 (2.4-2.5) | 4.4 (3.9-4.4) |
| Tetradecanol | 28.1 (20.8-43.3) | 4.6 (4.5-4.8) | 2.4 (2.2-2.5) |
| Hexadecanol | 11.9 (11.1-11.8) | 56 (51.2-61.7) | 85 (78.4-93.5) |
| 2-Decanol | NRb | 934 (823.2-1078.5) | 1,090 (567.3-13,696.9) |
| 2-Undecanol | NR | 141 (125.9-159.0) | 162 (150.9-174.5) |
| 2-Dodecanol | NR | 41.3 (39.60-43.06) | 75.3 (67.9-84.5) |
| 2-Hexadecanol | NR | 350 (332.5-370.0) | 720 (628.1-844.2) |
| 1,2-Dodecanediol | NR | 998 (720.2-1,622.7) | 928 (742.6-1,237.3) |
| 1,10-Decanediol | 67.0 (58.9-77.6) | 425 (394.4-462.3) | 1,607 (1,377.2-1,927.5) |
| 1,16-Hexadecanediol | 10.3 (9.3-11.6) | 37.3 (33.9-41.5) | 16.3 (15.7-16.9) |
| 10-Undecene-1-ol | 9.9 (8.8-11.3) | 45 (40.7-50.1) | 36.1 (32.7-40.4) |
| 12-Hydroxydodecanoic | 99.0 (78.2-134.4) | NR | NR |
| 16-Hydroxyhexadecanoic | 7.4 (5.9-9.9) | NR | NR |
a
Km values were derived from a double-reciprocal plot of substrate concentration and enzyme activity. Correlation coefficient values were always greater than 0.990.
b
NR, no reaction with this substrate.