FIGURE 4.

Genetic interaction of er-105 with bak1-3 and bak1-5 in ER-mediated resistance against PcBMM. (A) PcBMM biomass of the indicated genotypes at 5 dpi. Fungal biomass was determined by qRT-PCR using specific primers of PcBMM β-TUBULIN and Arabidopsis UBC21. Values were normalized to Arabidopsis UBC21 and are represented as the average (±SE) of the fold increase compared with the wild-type plants. These data represent average values of two replicates from one out of three independent experiments that gave similar results. Letters indicate groups statistically different (ANOVA, p < 0.05; Bonferroni test). (B) Macroscopic symptoms of the inoculated genotypes at 11 dpi (C) Immunoblot analyses of phosphorylated MAPKs (MPK6 and MPK3) after treatment of 12-days-old seedlings of the indicated genotypes with an extract of PcBMM spores. Phosphorylation was determined at the indicated time points by using the anti-pTEpY antibody. Comassie blue (CBB)-stained membranes show equal loading. (D) Relative expression of defense-related genes in 12-days-old seedlings from the indicated genotypes 1 h post-treatment with an extract of PcBMM spores (black boxes) or water (mock, white boxes). Relative expression levels to the UBC21 (At5g25760) gene are shown. Values are means (±SE) of three biological replicates (n = 6). Asterisks indicate significant differences compare to wild type plants values (Student’s t-test analysis, p < 0.05).